The only biochemically characterized member of the GHRC subfamily – enzyme from a methylotroph Methylobacterium extorquens (DHGY_METEA) was shown to reduce hydroxypyruvate and glyoxylate, and to catalyze the reverse reaction with glycerate. It was proposed to play a central role in the assimilation of carbon in methylotrophs as it converts hydroxypyruvate to glycerate (a key step in the serine cycle). The enzyme was shown to utilize both NADH and NADP as the cofactor. However, it is not known to what extent the characteristics of this enzyme apply to other members of this subfamily.
Accession Protein name Subfamily Organism Kingdom Kinetically characterized substrates Highest efficiency substrate Structures
Q8A8G0_BACTN glycerate dehydrogenase GHRC Bacteroides thetaiotaomicron Bacteria - - -
Q0PBD7_CAMJE 2-hydroxyacid dehydrogenase (EC: GHRC Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819 Bacteria - - -
Q97F10_CLOAB glycerate dehydrogenase GHRC Clostridium acetobutylicum ATCC 824 Bacteria - - -
Q24YV7_DESHY hypothetical protein GHRC Desulfitobacterium hafniense Y51 Bacteria - - -
Q72C72_DESVH glycerate dehydrogenase GHRC Desulfovibrio vulgaris Hildenborough Bacteria - - - 5TX7
Q8REX0_FUSNN D-3-phosphoglycerate dehydrogenase (EC: GHRC Fusobacterium nucleatum subsp. nucleatum ATCC 25586 Bacteria - - -
Q74CK1_GEOSL hprA; hydroxypyruvate reductase GHRC Geobacter sulfurreducens PCA Bacteria - - -
Y1556_HAEIN glycerate dehydrogenase (EC: GHRC Haemophilus influenzae Rd KW20 (serotype d) Bacteria - - -
O24922_HELPY 2-hydroxyacid dehydrogenase (EC: GHRC Helicobacter pylori 26695 Bacteria - - -
Q03XJ7_LEUMM 2-hydroxyacid dehydrogenase GHRC Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 Bacteria - - -
Q6LYW5_METMP glycerate dehydrogenase (EC: GHRC Methanococcus maripaludis S2 Archaea - - -
DHGY_METEA Glycerate dehydrogenase GHRC Methylobacterium extorquens Bacteria hydroxypyruvate, glyoxylate, glycerate hydroxypyruvate PMID:1657886
A0A0U1RH27_NEIMA glycerate dehydrogenase (EC: GHRC Neisseria meningitidis Z2491 (serogroup A) Bacteria - - -
D4GIZ2_PANAM hprA; HprA GHRC Pantoea ananatis LMG 20103 Bacteria - - -
D4GFK1_PANAM yiaE; YiaE GHRC Pantoea ananatis LMG 20103 Bacteria - - -
D5EYK0_PRER2 hprA; glycerate dehydrogenase (EC: GHRC Prevotella ruminicola Bacteria - - -
Q9HVG5_PSEAE hprA; glycerate dehydrogenase (EC: GHRC Pseudomonas aeruginosa PAO1 Bacteria - - -
Q9KP72_VIBCH D-lactate dehydrogenase GHRC Vibrio cholerae O1 biovar El Tor N16961 Bacteria - - -