Formate dehydrogenases (FDHs) represent a highly conserved subfamily of enzymes, characterized by a scattered taxonomic distribution. They are present in various bacteria (i.e., Firmicutes and proteobacteria) and eukaryotes (plants, yeasts, and fungi), and catalyze the NAD+-dependent oxidation of formate to carbon dioxide. The aspartate residue that defines the specificity towards NAD+ is conserved within the subfamily. FDHs play a pivotal role in methanol utilization in methylotrophic microorganisms (yeast and bacteria), supplying them with energy and reducing equivalents. As opposed to microbiological FDHs which function in cytoplasm, plant FDHs localize to the mitochondria and are key players in the cell stress response caused by both exogenic and endogenic factors.
Accession Protein name Subfamily Organism Kingdom Kinetically characterized substrates Highest efficiency substrate Structures
FDH_ARATH FDH; formate dehydrogenase FDH Arabidopsis thaliana (thale cress) Eukaryotes formate formate 10.1016/S0176-1617(00)80186-8 3N7U (NAD, azide ion)
ang:ANI_1_76134 An15g00410; formate dehydrogenase FDH Aspergillus niger Eukaryotes - - -
Q7VY50_BORPE formate dehydrogenase (EC: FDH Bordetella pertussis Tohama I Bacteria - - -
FDH_CANBO Formate dehydrogenase FDH Candida boidinii Eukaryotes formate formate PMID:27100912 2FSS PMID:17525463
2J6I PMID:17525463
A0A0A1EQY0_CANBO Formate dehydrogenase FDH Candida boidinii Eukaryotes - - - 5DN9 (NAD, azide ion) PMID:27100912
5DNA PMID:27100912
FDH_CHATD Formate dehydrogenase FDH Chaetomium thermophilum Eukaryotes formate formate 10.1016/j.molcatb.2015.09.014
G8NVB5_GRAMM Formate dehydrogenase FDH Granulicella mallensis Bacteria - - - 4XYB (NADP, azide ion) PMID:26104866
4XYE (NAD) PMID:26104866
4XYG PMID:26104866
Q5ZYS8_LEGPH formate dehydrogenase (EC: FDH Legionella pneumophila subsp. pneumophila Philadelphia 1 Bacteria - - -
O08375_MORSP Formate dehydrogenase FDH Moraxella sp. Bacteria - - - 2GSD (NAD, azide ion) PMID:19966418
3FN4 PMID:19966418
Q0DC43_ORYSJ Os06g0486800 FDH Oryza sativa japonica (Japanese rice) (RefSeq) Eukaryotes - - -
FDH2_ORYSJ Os06g0486900 FDH Oryza sativa japonica (Japanese rice) (RefSeq) Eukaryotes - - -
FDH_PSESR Formate dehydrogenase FDH Pseudomonas sp. Bacteria formate formate PMID:8484798 2GO1
2GUG (Formic acid)
2NAC PMID:8114093
2NAD (NAD, azide ion) PMID:8114093
FDH1_YEAST FDH1; formate dehydrogenase (NAD+) (EC: FDH Saccharomyces cerevisiae (budding yeast) Eukaryotes formate formate PMID:12144528
Q930E7_RHIME formate dehydrogenase (EC: FDH Sinorhizobium meliloti 1021 Bacteria - - -
sau:SA0171 fdh; formate dehydrogenase (EC: FDH Staphylococcus aureus subsp. aureus N315 (MRSA/VSSA) Bacteria - - -
Q76EB7_9PROT Formate dehydrogenase FDH Thiobacillus sp. KNK65MA Bacteria - - - 3WR5 (NAD)